Reticulocyte Dye Rau Sysmex Hematology Analyzers

Reticulocyte Dye rau Sysmex Hematology Analyzers muab cov phiaj xwm fluorescent nucleic acid staining rau semiconductor laser flow cytometry platforms. Cov kua reagent nkag mus rau hauv cov ntshav nucleated, reticulocytes, thiab platelets los khi xaiv nrog intracellular RNA thiab DNA, tsim ib sab fluorescence thiab lub teeb tawg teeb liab hla cov kev tshawb nrhiav. Formulated rau ncaj qha kev koom ua ke nrog automated fluidic systems, cov tshuaj tswj ruaj khov fluorescence siv, tsis tshua muaj suab nrov tom qab, thiab reproducible ntau ntau - mus rau - ntau yam kev ua tau zoo nyob rau hauv cov chaw kuaj mob.

Automated hematology dye reagents yog cov kua fluorescent cov cim ua haujlwm rau kev ntws -cytometry- raws li kev txheeb xyuas ntawm tes. Tsis zoo li cov ntawv qhia ntshav smear stains siv nyob rau hauv lub teeb microscopy, cov kua dyes nkag mus rau lub hom phiaj los ntawm kev tswj cov membrane permeabilization rau daim ntawv lo intracellular nucleic acids. Nyob rau hauv semiconductor laser excitation nyob rau hauv lub optical flow cell, daim ntawv sau npe cellular qauv emit sab fluorescence thiab lub teeb scatter signals, tso cai rau optics system ua kom meej meej ob-dimensional scattergrams thiab cell cluster distributions.

Hauv kev tsom xam kho qhov muag hematology, fluorescent dyes pab kom muaj kev sib txawv ntawm cov xov tooj ntawm tes los ntawm kev txheeb xyuas intracellular nucleic acid ntom:

Ntshav Cell Differential:Sib cais lymphocytes, monocytes, neutrophils, eosinophils, thiab basophils raws li RNA / DNA piv thiab cytoplasmic qauv.

Atypical & Immature Cell Identification:Txheeb xyuas cov blast cells thiab cov granulocytes tsis paub qab hau los ntawm kev siv cov fluorescence emissions.

Reticulocyte Enumeration:Kev ntsuas qhov seem ribosomal RNA hauv cov qe ntshav liab los ntsuas cov pob txha pob txha erythropoietic kev ua haujlwm.

Optical Platelet suav:daws cov platelets loj thiab sib txawv ntawm cov qe ntshav liab los ntawm cov platelets siv lub hom phiaj nucleic acid fluorescence gating.

Cov fluorescent dye ua haujlwm hla cov kab ke kho qhov muag tshwj xeeb:

WNR Channel:Suav cov qe ntshav dawb thiab cais cov qe ntshav liab nucleated los ntawm kev sib txawv nucleic acid staining.

WDF Channel:Sib txawv ntawm cov neeg laus leukocyte subpopulations thiab pom cov granulocytes tsis paub tab siv sab fluorescence thiab sab tawg tsis ua haujlwm.

WPC Channel:Cov ntxaij vab tshaus rau progenitor hlwb thiab txawv txav lymphocytes raws li nuclear ntom thiab nyias nyias permeability.

RET Channel:Ntsuas cov feem ntawm reticulocyte thiab kho qhov muag ntawm cov qe ntshav liab los ntawm RNA fluorescence siv.

PLT Channel:Labels platelet nucleic acids tshem tawm microcytic cov ntshav liab cuam tshuam thaum lub sijhawm ntsuas qhov muag.

Formulated rau kev ua haujlwm ncaj nraim nrog automated hematology platforms siv semiconductor laser flow cytometry:

XN Series:XN-1000, XN-2000, XN-3000, XN-9000, XN-L Series (XN-350, XN-450, XN-550)

XS Series:XS-500i, XS-800i, XS-1000i

XT Series:XT-1800i, XT-2000i, XT-4000i

XE Series:XE-2100, XE-5000

WNR Channel Staining (STAC-DYE-SYSWNR):Permeabilizes cell daim nyias nyias kom tso cai sai nuclear dye nkag, cais cov NRBC nuclei tsis zoo los ntawm cov qe ntshav dawb kom meej NRBC % thiab WBC suav tiam.

WDF Channel Staining (STAC-DYE-SYSWDF):Stains intracellular nucleic acids ntawm leukocytes los tsim cov pawg sib txawv rau cov neutrophils, lymphocytes, monocytes, thiab eosinophils.

WPC Channel Staining (STAC-DYE-SYSWPC):Selectively stains moj tej tawg hlwb thiab txawv txav lymphocytes raws li lipid muaj pes tsawg leeg thiab nuclear ntom rau lwm yam cell flagging.

RET Channel Staining (STAC-DYE-SYSRET):Kev khi rau RNA residual hauv cov qe ntshav liab tsis paub qab hau, ua kom suav cov feem pua ​​​​ntawm tag nrho cov reticulocyte, suav kiag li, thiab tsis paub qab hau reticulocyte feem.

PLT Channel Staining (STAC-DYE-SYSPLT):Daim ntawv lo platelet nucleic acids los tiv thaiv microcytic cov ntshav liab cuam tshuam thaum lub sij hawm qis - suav optical platelet ntsuas.

Ntim Configurations:

• Cartridge cassettes: 2 × 12 mL
• Lub raj mis: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL

Cia kub:2℃txog 30℃(Tshuaj los ntawm lub teeb ncaj qha thiab khov)
Unopened Shelf Life:18 hli
Ntawm -Board Stability:60 hnub nyob rau hauv ambient kuaj tej yam kev mob

Hematology Diluent Solutions:Isotonic buffer cov kev daws teeb meem tswj cov ntim ntawm tes kev ncaj ncees thiab tswj cov dej conductivity thaum ntsuas lub cev.
Lyse Reagents rau Leukocyte Differential:Cov kev daws teeb meem uas xaiv lyse cov qe ntshav liab los npaj cov qe ntshav dawb rau kev tshuaj xyuas qhov muag.
Analyzer Cleaning Solutions:Enzymatic ntxuav cov kua tsim los tshem tawm cov protein ntau thiab cov dye residue los ntawm cov kab hauv cov kua dej thiab cov hlwb kho qhov muag.
Concentrated Sheath Fluids:Hydrodynamic fluids tsim los rau nruab nrab cov qauv ntws hauv cov khoom siv kho qhov muag pom.

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