Dye for Mindray 5-Part Hematology Analyzers muaj cov polymethine tshwj xeeb thiab nucleic acid fluorescent dyes uas khi tshwj xeeb rau cellular DNA thiab RNA. Cov reagents no sau cov qe ntshav dawb, reticulocytes, thiab platelets hauv cov xov tooj ntawm tes, ua kom muaj ntau qhov sib txawv ntawm qhov sib cais ntawm semiconductor laser teeb tawg thiab nrhiav kom pom fluorescence.
Dab tsi yog Hematology Stain / Dye Reagents?
Hematology stain / dye reagents rau automated analyzers yog cov tshuaj tov tshuaj uas muaj cov organic fluorophores, surfactants, thiab osmotic stabilizers. Tsis zoo li phau ntawv microscopic ntshav smear stains, cov kua dye reagents ua haujlwm hauv kaw -fluidic automated flow cytometry systems.
Thaum txhaj tshuaj rau hauv cov tshuaj tiv thaiv chamber, cov dye permeates cell membranes npaj los ntawm cov lyse reagents tshwj xeeb thiab khi stoichiometrically rau intracellular nucleic acids. Thaum raug lub semiconductor laser beam nyob rau hauv lub ndlwg ntawm tes, cov fluorophores khi emit lateral fluorescent teeb proportional rau cellular nucleic acid cov ntsiab lus.
Lub luag haujlwm hauv Cell Classification / Detection
Fluorescent dye reagents pab kho qhov muag sib txawv ntawm cov ntshav cell complex:
Nucleic Acid Labeling:Fluorophores intercalate rau hauv intracellular RNA thiab DNA strands. Cov cell uas muaj kev ua haujlwm metabolic ntau dua lossis theem tsis paub qab hau nqus cov xim ntau dua, ua rau muaj zog fluorescent teeb liab.
Scattergram tiam:Kev sib xyaw ua ke ntawm lub teeb hluav taws xob sab nraud nrog kev siv fluorescence lateral tsim ntau -scattergrams.
Kev kuaj kab mob txawv txav:Distinct fluorescent emission profiles cais cov atypical lymphocytes, tsis paub tab granulocytes, thiab cov qe ntshav liab nucleated los ntawm cov qe ntshav dawb paub tab.
Kev Tshawb Nrhiav Channel
|
Kev Tshawb Nrhiav Channel |
Channel Name / Lub Hom Phiaj |
Biological Function & Measurement Method |
|
WNR Channel |
Dawb Cell Nucleated & RBC Channel |
Ntsuas tag nrho WBC suav thiab suav NRBC. Basophils tau sib txawv ntawm lub cev tawg thaum NRBC nucleic acids fluorescently sau npe. |
|
WDF Channel |
White Cell Differential Channel |
Sib txawv Neutrophils, Lymphocytes, Monocytes, thiab Eosinophils. Kev ntsuas qhov tsis paub qab hau Granulocytes los ntawm nucleic acid fluorescent zas xim khi kev siv zog. |
|
WPC Channel |
Dawb Cell Precursor Channel |
Cov tshuaj ntsuam rau cov hlwb progenitor, lymphocytes txawv txav, thiab blasts. Siv tshwj xeeb membrane permeability cov tshuaj tiv thaiv ua ke nrog dye uptake kom paub qhov txawv blast hlwb. |
|
RET Channel |
Reticulocyte Channel |
Kev ntsuas Reticulocyte piv, suav tsis txheeb, tsis paub tab Reticulocyte Fraction, thiab cov qe ntshav liab sib npaug ntawm RNA fluorescent staining. |
|
PLT Channel |
Platelet Optical Channel |
Muab optical platelet suav siv fluorescent dye labeling ntawm platelet RNA, tiv thaiv kev cuam tshuam los ntawm microcytic RBCs lossis khib nyiab. |
Compatible Analyzer Series
Automated 5-Part Laser Hematology Series:Formulated rau fluidic systems siv semiconductor laser fluorescence flow cytometry.
Siab -Throughput Modular Hematology Workstations:Tau tshaj lub hau automated -piercing samplers thiab nruam loading fluidic kab.
Integrated Hematology-Slide Maker Systems:Validated rau direct inline reagent xa tshuab yam tsis muaj teeb liab drift los yog siab variation.
Khoom Specifications
|
Parameter |
Technical Standard / Cov ntaub ntawv |
|
Hom qauv |
Concentrated kua fluorescent zas xim |
|
Active Fluorophore Base |
Polymethine / Nucleic acid fluorescent zas xim |
|
Excitation Wavelength |
633nm - 635nm (Liab semiconductor laser) |
|
Kev ntsuas pH (25 degree) |
7.00 – 8.50 |
|
Conductivity (25 degree) |
1.20-2.50 mS/cm |
|
Ceev (20 degree) |
1.005 - 1.020 g / cm³ |
|
Qhib -Vial Stability |
60 hnub (2℃- 30℃hauv qhov tsaus nti) |
|
Unopened Shelf Life |
18 hli |
|
Cia Kub |
2℃- 30℃(tiv thaiv los ntawm lub teeb thiab khov) |
|
Particulate Filtration |
Lim los ntawm 0.2 µm membrane |
Daim ntawv thov & Reagent muaj nuj nqi
WNR Differential Analysis:Txhaj rau hauv WNR cov tshuaj tiv thaiv chamber kom sau cov qe ntshav liab nucleated thiab cov qe ntshav dawb tom qab cytoplasm dissolution.
WDF 5-Part WBC Sib cais:Kev cuam tshuam nrog WBC subpopulations hauv WDF channel, tsim cov fluorescence sib txawv rau lymphocytes, monocytes, neutrophils, thiab eosinophils.
Precursor & Blast Cell Flagging:Formulated los xaiv nkag mus hloov pauv lipid daim nyias nyias hauv WPC channel, tso cai rau kev txheeb xyuas cov neeg tsis paub tab ntawm tes.
Reticulocyte RNA Quantification:khi rau cytoplasmic ribosomal RNA nyob rau hauv reticulocytes, tso cai rau xam cov theem ntawm reticulocyte maturation.
Optical Platelet suav:Stains sab hauv RNA fragments ntawm cov ntshav platelets los tsim kho qhov muag suav nyob rau hauv qis -platelet kev kho mob.
Ntim / Cia
|
Yam khoom |
Specification |
|
Txheem Ntim |
12 mL x 1 fwj / lub thawv, 42 mL x 1 lub raj mis / lub thawv |
|
Khoom siv lub raj mis |
Lub teeb-shielded amber fluorinated HDPE fwj |
|
Cia Kub |
2℃rau 30 degree |
|
Kev thauj mus los |
Tiv thaiv los ntawm khov thiab ncaj qha tshav ntuj |
Hais txog Hematology Reagents
Diluent Solution rau 5-Part Hematology Analyzers:Isotonic conductive buffer tov rau cov qauv ntshav dilution thiab tom qab lub suab nrov.
Lyse Reagent rau 5-Part Hematology Analyzers:Surfactant -raws li lysing tov rau xaiv erythrocyte membrane dissolution.
Concentrated Cleaning Solution:Lub tshuab ua kua rau kev siv cov kua dej tsis tu ncua thiab tshem tawm cov protein tso nyiaj.
Probe Cleanser:Alkaline hypochlorite sojntsuam ntxuav reagent rau cov qauv sojntsuam deproteinization.
FAQ
Q: Yuav ua li cas yog ib qho automated fluorescent dye reagent txawv ntawm cov ntshav smear stain reagents?
A: Ib txwm smear stains yuav tsum tau phau ntawv ziab ntawm cov iav slides rau kev ntsuam xyuas pom nyob rau hauv lub tshuab tsom. Automated analyzer dye reagents yog aqueous fluorescent dye concentrates tsim rau inline cov tshuaj tiv thaiv chav. Lawv khi rau cellular RNA / DNA nyob rau hauv cov kua ncua kev kawm ntawv, emitting lub teeb pom kev los ntawm laser flow cytometry optics.
Q: Puas yog lub teeb raug cuam tshuam rau kev ua haujlwm dyeing thaum ua haujlwm txhua hnub?
A: Yog. Active fluorophores degrade nyob rau hauv ntev raug rau lub teeb ncaj qha los yog ambient UV teeb. Cov tshuaj reagents tau ntim rau hauv lub teeb- cov thawv ntim khoom uas muaj cov khoom thaiv kab mob thiab yuav tsum nyob hauv qhov kaw reagent compartment thaum lub sijhawm ua haujlwm.
Q: Dab tsi yuav tsum tau khaws cia kub thaum lub sij hawm thauj mus los thiab warehouses?
A: Khaws cov tshuaj reagents ntawm 2℃thiab 30 degree. Cia qis dua 0℃ua rau fluorophore nag lossis daus. Kub siab tshaj 35℃ua kom cov khoom muaj nqis degradation.
Q: Ntau npaum li cas - rau - ntau qhov sib xws cuam tshuam cov txiaj ntsig scattergram li cas?
A: Kev hloov pauv hauv cov xim xim ua rau cov kab sib txawv ntawm pawg, ua rau muaj qhov yuam kev. Txhua qhov ntau lawm tau dhau los ua pov thawj fluorophore purity, kuaj spectrofluorometric, thiab matrix txuam kom tswj tau qhov chaw zoo ib yam.
Q: Dab tsi ntim tsim yog siv rau kev sib txuas fluidic?
A: Dye reagents yog muab tso rau hauv cov fwj tshuaj fluorinated HDPE nrog lub hau adapters tsim los rau cov kab dej ncaj qha, tiv thaiv evaporation thiab paug.
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