Platelet Dye Rau Sysmex Hematology Analyzers

Platelet Dye rau Sysmex Hematology Analyzers siv siab -purity fluorochrome chemistry kom xa cov xov tooj ntawm tes kom meej rau cov xov tooj ntawm tes nrhiav pom. Cov formulation permeates cellular daim nyias nyias nyob rau hauv cov tshuaj tiv thaiv chamber, khi tshwj xeeb rau intracellular nucleic acids nyob rau hauv semiconductor laser excitation. Cov tshuaj tiv thaiv no txo ​​qis kev cuam tshuam keeb kwm yav dhau los thaum tswj kev ruaj khov kho qhov muag scattergrams thoob plaws siab - dhau los ntawm kev kuaj mob.

Automated hematology dye reagents yog cov kev daws teeb meem fluorochrome engineered rau 5-feem thiab 6-ib feem sib txawv ntshav ntsuas kev ua haujlwm nrog semiconductor laser flow cytometry. Tsis zoo li phau ntawv microscopic ntshav smear stains, cov kua tshuaj dye formulations permeate cellular daim nyias nyias nyob rau hauv lub ntsuas tshuaj tiv thaiv chamber los khi ncaj qha nrog intracellular RNA thiab DNA. Thaum laser excitation, qhov tshwm sim fluorescent teeb emission ntsuas cellular qauv, ntim, thiab nucleic acid cov ntsiab lus.

Hauv laser flow cytometry, fluorescent dye reagents muab qhov sib piv los daws cov kab mob sib tshooj hauv cov ntshav:
Nucleic Acid Binding:Permeates leukocyte thiab reticulocyte daim nyias nyias los khi intracellular RNA thiab DNA qauv.
Fluorescence Intensity Differentiation:Distinguishes paub tab erythrocytes los ntawm reticulocytes thiab laus platelets los ntawm cov tsis paub tab platelet feem raws li nucleic acid ntom.
Cell Debris Separation:Tiv thaiv tsis tau -kev khi tshwj xeeb, tiv thaiv micro- cov hlab ntsha, cov qe ntshav liab tawg, thiab cov platelets loj los ntawm kev suav suav.
Atypical Cell Flagging:Tsim ib sab sib txawv - fluorescence signals cais cov lymphocytes txawv txav, blast cells, thiab nucleated cov ntshav liab.

Automated optical channels yuav tsum tau tshwj xeeb fluorochrome dye formulations rau tag cellular hom phiaj:
PLT Channel:Stains intracellular RNA nyob rau hauv platelets kom sib txawv ntawm erythrocyte cov khib nyiab thiab microcytes ntawm optical fluorescence, tshem tawm ntim-raws li suav yuam kev.
WNR Channel:Xaiv lyses cov qe ntshav liab thaum khaws cia cov leukocyte nuclei thiab cov qe ntshav liab nucleated rau tib lub sijhawm suav thiab kev faib tawm.
WDF Channel:Stains cytoplasmic thiab nuclear RNA / DNA ntawm neutrophils, lymphocytes, monocytes, thiab eosinophils los daws 5-qib leukocyte sib txawv.
WPC Channel:Sib txawv cov qe ntshav dawb ib txwm los ntawm cov hlwb tsis paub tab ua ntej raws li cov lipid membrane permeability thiab cov kev hloov pauv hauv nuclear.
RET Channel:Cim npe residual ribosomal RNA nyob rau hauv tsis paub qab hau erythrocytes kom quantify reticulocyte feem pua ​​thiab tsis paub qab hau reticulocyte feem.

Optical Platelet suav (PLT Channel)
Fluorochrome dye binds intracellular RNA, cais cov platelets los ntawm cov qe liab me me los yog cov khoom ntawm tes hauv cov qauv ntshav.

5-Pib Leukocyte Differential (WDF Channel)
Diffuses rau hauv cov qe ntshav dawb los khi cov nucleic acids, tsim cov teeb liab fluorescent variations uas cais cov lymphocytes, monocytes, neutrophils, thiab eosinophils.

Nucleated Red Blood Cell Isolation (WNR Channel)
Targets cov khoom siv nuclear hauv cov qe ntshav liab nucleated thiab cov qe ntshav dawb ib txhij, tiv thaiv cov qe ntshav liab nucleated los ntawm inflating tag nrho cov cell dawb.

Tsis paub Cell & Blast Screening (WPC Channel)
Permeates lipid daim nyias nyias ntawm cov blasts tsis paub tab thiab cov hlwb atypical los tsim cov pawg scattergram sib txawv thaum kuaj oncology.

Reticulocyte Analysis (RET Channel)
Stains residual RNA nyob rau hauv cov tub ntxhais hluas cov qe ntshav liab, ua rau kev ntsuas ntawm pob txha pob txha erythropoietic kev ua si.

Automated Lyse Reagents:Isotonic lysing cov kev daws teeb meem tsim rau kev hloov pauv hemoglobin sai thiab erythrocyte dissolution.

Diluent Solutions:High -purity buffered electrolyte cov kev daws teeb meem uas tswj cov cell ntim thiab ruaj khov conductivity.

Sheath Fluid:Tsawg-cov hauv qab laminar ntws cov kev daws teeb meem rau hydrodynamically tsom cov cell hauv optical flow cells.

Enzymatic Cleaning Solutions:Proteolytic ntxuav cov kev daws teeb meem tsim los tshem tawm cov protein deposits los ntawm txoj kev fluidic.

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